In this paper, Professor Feng Ren used the digital PCR product from TargetingOne as a reference standard for assay sensitivity and positive detection rate, which can also test the effectiveness.
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Droplet digital PCR (ddPCR), as a new and accurate quantitative technique, is an absolute quantitative method for the detection of genes (10, 11), especially for trace specimens (12–14).
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For the analytical method, nine laboratories employed one-step RT-dPCR approach using kits corresponding to the spe-cific dPCR platforms. One laboratory employed a two-step RT-dPCR approach, where (cDNA) was synthesized in a separate reaction tube.
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Single-dose intranasal administration of this vaccine completely protected human DPP4 knock-in mice from lethal MERS-CoV challenge. Passive transfer of immune sera also conferred a survival advantage in lethal challenge mouse models (32).
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To determine the LoDs of diagnostic assays, various reference materials such as synthetic RNA transcripts, extracted genomic RNA, armored RNA, or inactivated virus particles with known concentrations or titers are commonly used.
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